Peculiarities of phenotypes of patients with pyelo- and glomerulonephritis by HLA distribution analysis

  • V. Driianska SI “Institute of Nephrology NAMS of Ukraine”
  • O. Petrina SI “Institute of Nephrology NAMS of Ukraine”, SI “Institute of Urology NAMS of Ukraine”
  • M. Velychko SI “Institute of Nephrology NAMS of Ukraine”
  • F. Haisenyuk Shupyk National Medical Academy of Postgraduate Education
  • G. Drannik SI “Institute of Urology NAMS of Ukraine”
Keywords: HLA-phenotype, pyelonephritis, glomerulonephritis, nephrotic syndrome, causal role, antigen-protector


Studies devoted to the role of human leucocyte antigens (HLA) in pathogenesis of chronic kidney disease (CKD) have demonstrated the associative links of the HLA antigens, which stipulate the relative and attributive risks of some autoimmune diseases, with immune disorder and a high production of pro-inflammatory cytokines.

The aim of our study was to determine the peculiarities of phenotypes of CKD patients according to the distribution of HLA-A, B and DR antigens and to conduct their comparative analysis in patients with pyelonephritis (PN) and glomerulonephritis (GN).

Methods: The distribution of HLA-A, B, DR antigens in 384 CKD patients (120 with PN and 264 with GN) was analyzed. HLA antigens were defined using a standard microlymphocytotoxic test on the Terasakiґs planchette with special panels of anti-HLA serums (20 antigens of locus A, 31 – B and 9 – DR). The control group consisted of 350 healthy donors.

The HLA antigen frequencies in normal and diseased subjects were compared taking each antigen separately, using χ2 test. The etiologic fraction (attributive risk s > 0,1) was counted using the formula: s = x - y/I- y, where x is frequency of antigen in patients and y is frequency in healthy. The s  reading was considered reliable when it exceeded 0.1.

Results. The causal role (σ > 0,1) was determined for А10, А11; В14, В16 for PN; antigens-protectors - А2, В21, В35, В40.

For CGN, NS the relative risk is high (RR > 2) at the presence of HLA-A23, А24, А28; B8, В38, В41, В44; DR1, DR4, DRw52 in phenotype, the causal role in etiopathology (σ>0.1) is indicated for A24,А28; B8; DR1, DR4, DRw52; the disease protectors are B12 and B16.

Conclusion. Conclusion. The features of the HLA-phenotype of patients with pyelo- and glomerulonephritis were shown. It allowed to establish the interconnectedness of the antigens of the histocompatibility complex with the risk of kidney diseases developing, which could help to personificate of the treatment and predicte of the course of the disease.


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Genetic passport - the basis of individual and predictive medicine / ed. V.S. Baranov. SPb .: publishing house N-L. 2009; 528 р. [In Russian]. Available from:

Dmitrieva NG, Jakovchik ON, Vatazin AV., Zul’karnaev AB., Fedulkina VA. Histocompatibility system in renal transplantation / Almanac of Clinical Medicine. 2014; 83 (31): 83-87.  [In Russian]. doi. 10.18786/2072-0505-2014-31-83-87.

Erlich H. HLA DNA typing: past, present, and future. Tissue Antigens. 2012. 80 (1): 1-11. doi: 10.1111/j.1399-0039.2012.01881.x.  

Alekseev LP., Khaitova NM., Yazdovski V. HLA-associated predisposition to diseases and some mechanisms for its implementation. Bulletin of the USSR Academy of Medical Sciences.  1998;  № 5: 30 - 37. [In Russian].

Dausset J. Etat actuel de l'immunologie des leucocytes.  doi. 10.1111/j.1423-0410.1957.tb03697.x 

Zaretskaya Yu.M.  Clinical immunogenetics.   М. : Medicine:  1983. 208с  [In Russian]. Available from:

Shabalov NP. Diseases associated with HLA antigens. [In Russian]

Gavrilenko T., Minchenko G., Pidhaina O., Rigkova N.  Modern substantiation of the participation of immunopathological reactions and genetic predisposition to their development in patients with coronary heart disease. Immunology and allergology. 2011; № 1: 93-94. [In Ukrainian].

Gavrilyuk A.M.   The role of HLA-antigens in breach of reproductive function of a woman . Medical aspects of women's health. 2010. №2 (29): 42-9. [In Ukrainian]. Available from:

 Tsabolova Z., Sokolova Yu., Sizyakina L. Raspredeleniye HLA 1 klassa u bol'nykh êndemicheskim zobom. Cytokines and Inflammation. 2011; 10 (1): 6-8. [In Russian]. Available from:

 Shestakov AE.  Study of the association of a number of candidate genes with chronic glomerulonephritis. [dissertation]. PhD: Мoscow; 2006. 95p. [In Russian]. Available from:

Marsh S.G., Albert E.D., Bodmer W.F. , Bontrop R.E., Dupont B., Erlich H.A., Fernández-Viña M., Geraghty D.E., Holdsworth R., Hurley C.K., Lau M., Lee K.W., Mach B., Maiers M., Mayr W.R., Müller C.R., Parham P., Petersdorf E.W., Sasazuki T., Strominger J.L., Svejgaard A., Terasaki P.I., Tiercy J.M., Trowsdale J. Nomenclature for factors of the HLA system, 2010. Tissue Antigens. 2010; 75 (4): 291-455. doi:  [10.1111/j.1399-0039.2010.01466.x]

 Drannik G. Clinical immunology and allergology. Kiyv : Polygraph Plus. 2010. [In Russian]. Available from:  Available from: 

Schieppati A., Remuzzi, G. Chronic Renal Disease as a Public Health Problem: Epidemiology, Social, and Economic Implications. Kidney International Supplements. 2005; 68: 7-10. 
doi. 10.1111/j.1523-1755.2005.09801.x 

Couser William G. and Johnson Richard J. The etiology of glomerulonephritis: roles of infection and autoimmunity. Kidney International. 2014; 86: 905–14. doi: 10.1038/ki.2014.49.

 Dyadyk AI., Kolesnik MO. Infections of the kidneys and urinary tract. Donetsk: Region; 2003. [In Russian].

Fogo AB.  Nephrotic syndrome: Molecular and genetic basis. Nephron. 2000; 85 (1) : 8-13. doi: 10.1159/000045623.

 Gluhovschi  C. What is the significance of HLA-DR antigen expression in the extraglomerular mesangium in glomerulonephritis. Hum Immunol. 2012; 73 (11): 1098-101. doi: 10.1016/j.humimm.2012.07.326

 Tsibulkin AP, Hasanova MI, Sitkina KV. Phenotype of peripheral blood lymphocytes in patients with glomerulonephritis: clinical and morphofunctional relationships. Immunology.  2010; 1 (1): 34-7. [In Russian].  Available from:

 Kim AN, Markiewicz MA, Shaw AS.   New roles reveled for T cells and DCs in glomerulonephritis. J Clin Invest. 2009; 119 (5): 1074–76. doi:  10.1172/JCI39071.

Drannik GN, Maidannik VG. Klinicheskoye znacheniye tipirovaniya antigenov sistemy HLA u bol'nykh piyelonefritom i glomerulonefritom. Vrachebnoye delo. 1988; №4: 9-12. [In Russian].

Hill  AV. The immunogenetics of human infectious diseases. Annu Rev Immunol. 1998;16: 593-617. Available from:

Rashid H,  Papiha S, Agroyannis B. The association of HLA and other genetic marckers with glomerulonephritis. Hum Genet. 1983; 63: 38-44. Available from:

Popov EA. Immunogenetic aspects of chronic liver diseases (pathogenesis, clinic, diagnosis) [dissertation]. PhD: Astrakhan Medical Academy; 2004. [In Russian]. Available from:

Gough SC, Simmonds MJ. The HLA Region and Autoimmune Disease: Associations and Mechanisms of Action. Curr Genomics. 2007;8(7):453-65. doi: 10.2174/138920207783591690

Crux NB, Elahi S. Human Leukocyte Antigen (HLA) and Immune Regulation: How Do Classical and Non-Classical HLA Alleles Modulate Immune Response to Human Immunodeficiency Virus and Hepatitis C Virus Infections?. Front Immunol. 2017;8:832. Published 2017 Jul 18. doi:10.3389/fimmu.2017.00832

Drannik GN, , Montag TS,  Zolotkovskaya OZ. Antigen HLA-B8 kak vozmozhnyy faktor riska razvitiya zabolevaniy, soprovozhdayushchikhsya autoimmunnym komponentom. Urologiya i nefrologiya. 1988; № 6: 20-23. [In Russian].

Kolesnik MO., Driyanska VЕ, Velichko MB, Drannik GM, Petrina OP, Nepomnyashchy VM. Association of HLA and proinflammatory cytokines of blood in patients with chronic glomerulonephritis. Ukrainian Journal of Nephrology and Dialysis. 2017; № 1: 35-41. [In Ukrainian]. doi: 10.31450/ukrjnd.1(53).2017.06

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How to Cite
Driianska, V., Petrina, O., Velychko, M., Haisenyuk, F., & Drannik, G. (2018). Peculiarities of phenotypes of patients with pyelo- and glomerulonephritis by HLA distribution analysis. Ukrainian Journal of Nephrology and Dialysis, (4(60), 11-18.