Evaluation of protective effects of melatonin on free radical metabolism in rat kidney during ischemia-reperfusion

M.  Nisari; A.  Yay; T.  Ertekin; M.  Nisari; Ö.  Al; D.  Ceylan; G. Ö.  Önder; M.  Kavutcu

doi:10.31450/ukrjnd.4(64).2019.04

M. Nisari Nuh Naci Yazgan University Faculty of Health Sciences https://orcid.org/0000-0001-7469-8921
A. Yay Erciyes University Medical Faculty https://orcid.org/0000-0001-8532-3543
T. Ertekin Kocatepe University School of Medicine https://orcid.org/0000-0003-1756-4366
M. Nisari Erciyes University Medical Faculty https://orcid.org/0000-0002-1126-7478
Ö. Al Erciyes University Medical Facult https://orcid.org/0000-0001-5292-3593
D. Ceylan Erciyes University https://orcid.org/0000-0003-3622-2249
G. Ö. Önder Erciyes University Medical Faculty https://orcid.org/0000-0002-0515-9286
M. Kavutcu Gazi University Medical Faculty https://orcid.org/0000-0002-5135-8067

DOI: https://doi.org/10.31450/ukrjnd.4(64).2019.04

Keywords: rat, melatonin, ischemia, reperfusion

Abstract

The purpose of this research was to investigate the possible protective effect of melatonin, as a potent antioxidant on I/R-induced renal injury in rats.

Methods. We used 28 female Wistar albino rats weight 200-250g. The rats were randomly divided into 4 groups. Control Group (C): They were fed with only standard rat diet and tap water without drug injections or ischemia-reperfusion. Melatonin Group (M): 25 mg/kg melatonin was administered i.p 30 min. Ischemia/Reperfusion Group (I/R): Rats were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion. Melatonin+ischemia/reperfusion Group (M+I/R): Melatonin (25 mg/kg) was administered 30 min prior to ischemia and immediately before the reperfusion period. Rats were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion.

Results. While MDA levels increased in the I/R group, SOD and GST activities were seen to be significantly increased. Although the increase of the SOD activity was observed in the M+I/R group, no meaningful difference was found. MDA levels were significantly decreased in M+I/R group compared to the control group, CAT and GST activities were significantly increased.

Conclusions. Our results show that the treatment with M may prevent kidney damage due to ischemia result in increasing oxidant stress peroxidation damages further. Melatonin or its metabolites are capable of neutralizing free radicals and non-radical oxygen-based reactants. This study suggests that melatonin may be an effective antioxidant agent.

Downloads

Download data is not yet available.

References

1. https://doi.org/10.1016/S0039-6109(16)45628-8" target="_blank" rel="noopener">Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am. 1992; 72: 65-83. doi: 10.1016/S0039-6109(16)45628-8.

2. Charles D.Collard, Simon Gelman. Pathophysiology, Clinical Manifestations, and Prevention of ischemia-reperfusion injury. Anesthesiology. 2001;94:1133-8. doi:10.1097/00000542-200106000-00030.

3 Conesa LE, Valero F, Nadal JC et al. N-acetyl-L-cysteine improves renal medullary hypoperfusion in acute renal failure. Am J Physiol. 2001; 281: 730-73. doi: 10.1152/ajpregu.2001.281.3.R730.

4. Reiter RJ. Oxidative processes and antioxidant defense mechanisms in the aging brain. FASEB J. 1995;9:526-533. doi: 10.1096/fasebj.9.7.7737461

5. Kurcer Z, Oguz E, Özbilge H, et al. Melatonin protects from ischemia/reperfusion-induced renal injury in rats: this effect is not mediated by proinflammatory cytokines. J Pineal Res. 2007;43:172-78. doi: 10.1111/j.1600-079X.2007.00459.x.

6. Jaeschke H. The inflammatory response after hepatic ischemia/reperfusion. In J. J. Lemaster, and C. Oliver (Eds.), Cell Biology of Trauma. Florida: CRC Press, 1995:127-42.

7. Reiter RJ, Melchiorri D, Sewerynek E, et al. Review of the evidence supporting melatonin’s role as an antioxidant. J Pineal Res. 1995;19:149-65. doi: 10.1111/j.1600-079X.1995.tb00133.x.

8. Lerner AB, Case JD, Takahashi Y, et al. Isolation of melatonin, the pineal gland factor that lightens melanocytes. J Amer Chem Soc. 1958;80:2587.doi: 10.1021/ja01543a060.

9. Cassone VM, Chesworth MJ, Armstrong SM. Entrainment of rat circadian rhythms by daily injection of melatonin depends upon the hypothalamic suprachiasmatic nuclei. Physiol Behav. 1986;36:1111-21. doi: 10.1016/0031-9384(86)90488-9.

10. Altun A, Vardar A, Altun B.U. Melatonin ve Kardiyovasküler Sistem. nadolu Kardiyoloji Dergisi. 2001;1:283-88.

11. Yildirim Z, Kotuk M, Erdogan H, et al. Preventive effect of melatonin on bleomycin-induced lung fibrosis in rats. J Pineal Res. 2006;40:27-33. doi: 10.1111/j.1600-079X.2005.00272.x.

12. Aydoğdu N, Kanter M, Erbaş H, et al. Kadmiyuma bağlı karaciğer hasarında taurin, melatonin ve asetilsisteinin nitrik oksit, lipid peroksidasyonu ve bazı antioksidanlar üzerindeki etkileri. Erciyes Tıp Dergisi. 2007;29:089-96.

13. Reiter RJ, Tan DX, Poeggeler B, et al. Melatonin as a free radical scavenger: Implications for aging and age-related diseases. Ann N Y Acad Sci. 1994;719:1-14. doi: 10.1111/j.1749-6632.1994.tb56817.x

14. De L Lastra CA, Cabeza J, Motilva V, et al. Melatonin protects against gastric ischemia-reperfusion injury in rats. J Pineal Res. 1997;23:47-52. doi: 10.1111/j.1600-079X.1997.tb00334.x

15. Baker GL, Autor AP, Corry RJ. Effect of allopurinol on kidneys after ischemia and reperfusion. Curr Surg. 1985;42:466-69.

16. Dillon JJ, Grossman SH, Finn WF. Effect of oxypurinol on renal reperfusion injury in the rat. Renal Failure. 1993;15:3745. doi: 10.3109/08860229309065570.

17. Torras J, Seron D, Herrero I et al. Renal protective effect of liposomal superoxide dismutase in an experimental warm ischemia model. Transpl Int. 1994;7: S472-S475. doi: 10.1111/j.1432-2277.1994.tb01421.x.

18. Alatas O, Sahin A, Colak O et al. Beneficial effects of allopurinol on glutathione levels and glutathione peroxidase activity in rat ischaemic acute renal failure. J Int Med Res. 1996; 24: 33-39. doi: 10.1177/030006059602400105.