Long-COVID sequelae are associated with oxidative stress in hemodialysis patients

N.  Stepanova; L.  Korol; L.  Snisar; A.  Rysyev; T.  Ostapenko; V.  Marchenko; O.  Belousova; O.  Popova; N.  Malashevska; M.  Kolesnyk

doi:10.31450/ukrjnd.1(77).2023.05

N. Stepanova State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine» https://orcid.org/0000-0002-1070-3602
L. Korol State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine» https://orcid.org/0000-0002-5484-0326
L. Snisar State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine» https://orcid.org/0000-0002-2968-0885
A. Rysyev Dialysis Medical Center LLC “Link-Medital”
T. Ostapenko Dialysis Medical Center LLC “Nephrocenter”
V. Marchenko Dialysis Medical Center LLC “Nephrocenter”
O. Belousova Dialysis Medical Center LLC “Nephrocenter”
O. Popova State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine»
N. Malashevska State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine»
M. Kolesnyk State Institution «Institute of Nephrology of the National Academy of Medical Sciences of Ukraine» https://orcid.org/0000-0001-6658-3729

DOI: https://doi.org/10.31450/ukrjnd.1(77).2023.05

Keywords: COVID-19, long-COVID sequelae, hemodialysis, oxidative stress.

Abstract

The pathophysiology of long-COVID sequelae in the general population of SARS-CoV-2-infected patients has been shown to be strongly influenced by oxidative stress. However, the potential role of oxidative stress in the development of long-COVID sequelae in hemodialysis patients (HD) has never been investigated.

The present study aimed to evaluate the oxidative status of HD patients 3.5 months after SARS-CoV-2 infection in relation to the presence of long-COVID sequelae and the severity of the acute phase COVID-19.

Methods. This cross-sectional cohort study included 63 HD patients with a median age of 55 (43-62.5) years and a dialysis vintage of 42 (25-73) months who had been infected with COVID-19 at least 3 months before recruitment. Patients were divided into two groups according to the occurrence of long-COVID sequelae: Group 1 included 31 (49.2%) HD patients with sequelae, while Group 2 included 32 (50.8%) fully recovered individuals. At 3.5 (3.2-4.6) months after the acute phase of COVID-19, malondialdehyde (MDA) and erythrocyte levels (MDAe), sulfhydryl groups (SH -groups), serum catalase activity, transferrin, and ceruloplasmin were measured. A comparison of the obtained data was performed using the Student’s test or the Mann-Whitney test according to the data distribution. A correlation was evaluated with the Spearman test.

Results. HD patients with persistent long-COVID sequelae had significantly higher concentrations of MDAs (p = 0.002), MDAe (p = 0.0006), and CTs (p = 0.02), and lower serum levels of SH-groups (p = 0.03) and ceruloplasmin (p = 0.03) compared with Group 2. The concentration of most studied indicators of pro- and antioxidant status did not depend on the severity of the acute phase COVID-19, and only catalase activity was statistically significantly related to the need for hospitalization (r = 0.59; p = 0.001), oxygen support (r = 0.44; p = 0.02), and the percentage of lung injury according to computed tomography (p = 0.03). Although the serum concentration of transferrin did not differ between the studied groups, the individual analysis showed that its value was statistically higher in HD patients with severe COVID-19 even 3.5 months after infection (p < 0.0001).

Conclusions. Long-term COVID-19 sequelae in HD patients are associated with oxidative stress. High levels of catalase activity and serum transferrin 3.5 months after COVID-19 may be a consequence of the severe course of the acute phase of the disease. The obtained data suggest that the use of antioxidants may be one of the possible strategies to treat the long-term consequences of COVID in HD patients.