The beneficial effect of a phytonutrient-rich product against cadmium chloride-induced hepatorenal toxicity

DOI: https://doi.org/10.31450/ukrjnd.4(72).2021.04
Keywords: hepatotoxicity, nephrotoxicity, trévo, oxidative stress, cadmium.

Abstract

Abstract. This study was designed to investigate the hepatorenal protective effects of trévo, on cadmium-induced renal and hepatic injury in male Wistar rats.

Methods. Fifteen healthy male Wistar rats were divided into three groups of five rats per group. Group I (control); group II (35mg/kg cadmium chloride (CdCl2); Group III (2 ml/kg trévo+ CdCl2. The rats were treated with trévo (2ml/kg orally) and administered CdCl2 3 hrs later. Twenty-four hours after the last administration rats were sacrificed and blood was collected via cardiac puncture and processed for hematological parameters and assessment of urea, creatinine (CREA), and uric acid (UA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (ALB). The liver and kidney were excised and processed for markers of oxidative stress.

Results intraperitoneal administration of 35 mg/kg of CdCl2 caused a significant increase in serum concentration of urea, CREA, UA, AST, ALT, while the concentration of ALB was significantly lower (P<0.0001). CdCl2 caused a significant reduction in packed cell volume, hemoglobin while the total white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were increased. Oxidative stress was significantly pronounced in the liver and kidney of rats exposed to CdCl2 as observed in the high concentration of malondialdehyde, decreased concentration of glutathione, the activity of catalase, superoxide dismutase, and glutathione-S-transferase. Pretreatment with trévo was able to significantly prevent the anemic, oxidative damage, renal and hepatic injury initiated by CdCl2.

Conclusions. The study reveals that trévo is effective in attenuating cadmium-induced hepatorenal toxicity in male Wistar rats.

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References

Bilen S, Bilen M, Turan, V. Relationships between cement dust emissions and soil properties. Pol. J. Environ. Stud. 2019;28: 3089–3098. doi: 10.15244/pjoes/92521.

Naeem I, Masood N, Turan V, Iqbal M. Prospective usage of magnesium potassium phosphate cement combined with Bougainvillea alba derived biochar to reduce Pb bioavailability in soil and its uptake by Spinacia oleracea L. Ecotoxicol. Environ. Saf. 2021; 208: 111723. doi: 10.1016/j.ecoenv.2020.111723.

Shahbaz A.K., Adnan, R.P.M., Saeed, R., Turan, V., Iqbal, M., Lewinska, K., Abbas, F., Saqib, M., Tauqeer, H.M., Iqbal, M., Fatima, M., Rahman, M.U. Effects of biochar and zeolite soil amendments with foliar proline spray on nickel immobilization, nutritional quality and nickel concentrations in wheat. Ecotoxicol. Environ. Saf. 2019; 173: 182–191. doi:10.1016/j.ecoenv.2019.02.025.

Turan V. Potential of pistachio shell biochar and dicalcium phosphate combination to reduce Pb speciation in spinach, improved soil enzymatic activities, plant nutritional quality, and antioxidant defense system. Chemosphere. 2020; 245: 125611. doi:10.1016/j.chemosphere.2019.125611.

Jiao D, Jian Q, Liu Y, Ji L. Nephroprotective effect of wogonin against cadmium-induced nephrotoxicity via inhibition of oxidative stress–induced MAPK and NF-kB pathway in Sprague Dawley rats. Human and Experimental Toxicology. 2019;9:1–10. doi:10.1177/0960327119842635.

Rafati RM, Kazemi S, Moghadamnia AA. Cadmium toxicity and treatment: An update. Caspian J Intern Med. 2017; 8(3): 135-145. doi:10.22088/cjim.8.3.135.

Waalkes MP. Cadmium carcinogenesis. Mutat Res. 2003; 533: 107-20. doi:10.1016/j.mrfmmm.2003.07.011.

Liu J, Qu W, Kadiiska MB. Role of oxidative stress in cadmium toxicity and carcinogenesis. Toxicol Appl Pharmacol. 2009; 238: 209-14. doi:10.1016/j.taap.2009.01.029.

Rani A, Kumar A, Lal A, Pant M. Cellular mechanisms of cadmium-induced toxicity: a review. Int J Environ Health Res. 2014; 24: 378-99. doi:10.1080/09603123.2013.835032.

Gabr SA, Alghadir AH, Ghoniem GA. Biological activities of ginger against cadmium-induced renal toxicity. Saudi Journal of Biological Sciences. 2019;26: 382–389. doi:10.1016/j.sjbs.2017.08.008.

Bekheet SH, Awadalla EA, Salman MM, Hassan MK. Bradykinin potentiating factor isolated from Buthus occitanus venom has a protective effect against cadmium induced rat liver and kidney damage. Tissue Cell. 2011 ;43: 337–343. doi:10.1016/j.tice.2011.07.001.

Deevika B, Asha S, Taju G, Nalini T. Cadmium acetate induced nephrotoxicity and protective role of curcumin in rats. Asian J. Pharm. Clin. Res. 2012; 5(3): 186–188. Available at: https://www.researchgate.net

El-Sharaky AS, Newairy AA, Badreldeen MM, Eweda SM, Sheweita SA. Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology. 2007;235: 185–193. doi: 10.1016/j.tox.2007.03.014.

Ognjanovic BI, Markovic SD, Ethordevic NZ, Trbojevic IS, Stajn AS, Saicic ZS. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: protective role of coenzyme Q(10) and vitamin E. Reprod. Toxicol. 2010; 29: 191–197. doi:10.1016/j.reprotox.2009.11.009.

Renugadevi J, Prabu SM. Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Exp. Toxicol. Pathol. 2010; 62: 171–181. doi:10.1016/j.etp.2009.03.010.

Akinmoladun AC, Oguntunde KO, Owolabi L, Ilesanmi OB, Ogundele JO, Olaleye MT, Akindahunsi AA. Reversal of acetaminophen-generated oxidative stress and concomitant hepatotoxicity by a phytopharmaceutical product. Food science and human wellness. 2017;6: 20-27. doi:10.1016/j.fshw.2016.11.001.

Ilesanmi OB, Atanu OF, Odewale TT, Adeogun E, Nnaemeka CB, Alaneme CU, Ogonye D, Ogbonna JC. Effect of a Phytonutrient-Rich Product and Administration Time on Cyanide-Induced Cardiotoxicity. Trop J Nat Prod Res. 2020; 4(7):304-309. doi :10.26538/tjnpr/v4i7.9.

Ilesanmi OB, Ikpesu T. Neuromodulatory activity of trèvo on cyanide-induced neurotoxicity viz neurochemical, antioxidants, cytochrome C oxidase and p53. Advanced Traditional Medicine. 2020. doi:10.1007/s13596-020-00450-w.

Jain N.C. Essential of Veterinary Hematology. Lea and Febiger, Philadelphia. 1993; р.133-168.

Varshney R, Kale RK. Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol.1990;58:733–43. doi:10.1080/09553009014552121.

Jollow DJ, Mitchell JR, Potter WZ, Davis DC, Gillette JR, Brodie BB. Acetaminophen-induced hepatic necrosis. II. Role of covalent binding in vivo. J Pharmacol Exp Ther. 1973; 187: 195–202.

Aebi H. Methods of Enzymatic Analysis (Second Edition). 1974;2:673-684. doi:10.1016/B978-0-12-091302-2.50032-3.

Misra HP, Fridovich I. The univalent reduction of oxygen by reduced flavins and quinones. J Biol Chem. 1972;247:188–192. doi:10.1016/S0021-9258(19)45773-6.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem.1974; 249:7130–7139. doi:10.1016/S0021-9258(19)42083-8.

Geng HX, Wang L. Cadmium toxic effects on placental and embryonic development. Environ. Toxicol. Pharmacol. 2019; 67:102–107. doi:10.1016/j.etap.2019.02.006.

Daley GM, Pretorius CJ, Ungerer JP. Lead toxicity, An Australian perspective. Clin Biochem Rev.2018; 39:61–98.

El‐Sayed YS, El‐Gazzar AM, El‐Nahas AF, Ashry KM. Vitamin C modulates cadmium‐induced hepatic antioxidants' gene transcripts and toxicopathic changes in Nile tilapia, Oreochromis niloticus. Environ Sci Pollut Res Int.2016;23:1664-1670. doi:10.1007/s11356-015-5412-8.

El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB. Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. Trace Elem. Medical. Biology. 2015; 29:104-110. doi:10.1016/j.jtemb.2014.05.009.

Wu P, Su C, Chang H, Lan A, Yang S. The Effect of a Nutritional Supplement on Chronic Kidney Disease Patients. Journal of Food and Nutrition Researchl.2016;4 (2):115-120. doi:10.12691/jfnr-4-2-8.

Sureshkumar D, Shamshad Begum S, Johannah NM, BaluMaliakel, Krishnakumar IM. Toxicological evaluation of a saponin-rich standardized extract of fenugreek seeds (FenuSMARTTM): Acute, sub-chronic and genotoxicity studies. Toxicology Reports journal. 2018;5:1060-1068. doi:10.1016/j.toxrep.2018.10.008.

Sanders AP, Mazzella MJ, Malin AJ, Hair GM, Busgang SA, Saland JM, Curtin P. Combined exposure to lead, cadmium, mercury, and arsenic and kidney health in adolescents age 12–19 in NHANES 2009–2014. Environ. Int. 2019;131:104993. doi:10.1016/j.envint.2019.104993.

Shatti AA. Effects of Origanum Majorana L on cadmium induced hepatotoxicity and nephrotoxicity in albino rats. Saudi medical journal.2011;32(8):797-805.

Satarug S, Vesey DA, Gobe GC. Current health risk assessment practice for dietary cadmium: data from different countries. Food and Chemical Toxicology. 2017;106:430-445. doi:10.1016/j.fct.2017.06.013.

Ohno I. Relationship between hyperuricemia and chronic kidney disease. Nucleosides, Nucleotides Nucl. Acids.2011;30:1039-1044. doi:10.1080/15257770.2011.611484.

Babatunde OI, Abigail A. Ameliorative effect of a multi-nutrient-rich product against cyanide induced hepatorenotoxicity. Drug Discovery.2021;15(35):51-59.

Haghighipour S, Soltan R, Anjomsho A. The protective effect of lycopene supplement against vancomycin-induced nephrotoxicity; a randomized double-blind placebo-controlled clinical trial. J Renal Inj Prev.2020;9(4):e32. doi:10.34172/jrip.2020.32.

Prabu SM, Shagirtha K, Renugadevi J. Naringenin in combination with Vitamin C and E partially protects oxidative stress-mediated hepatic injury in cadmium-intoxicated rats. J Nutr Sci Vitaminol.2011;57(2):177-185. doi:10.3177/jnsv.57.177.

Dkhil MA, Al-Quraishy S, Diab MM, Othman MS, Aref AM, Abdel Moneim AE. The potential protective role of Physalis peruviana L. fruit in cadmium-induced hepatotoxicity and nephrotoxicity. Food Chem Toxicol. 2014;74:98-106. doi:10.1016/j.fct.2014.09.013.

Varoni MV, Pasciu V, Gadau SD, Baralla E, Serra E, Palomba D, Demontis MP. Possible antioxidant effect of Lycium barbarum polysaccharides on hepatic cadmium-induced oxidative stress in rats. Environ Sci Pollut Res Int. 2017; 24(3):2946-2955. doi:10.1007/s11356-016-8050-x.

Ahmed RA. Hepatoprotective and antiapoptotic role of aged black garlic against hepatotoxicity induced by cyclophosphamide. The Journal of Basic and Applied Zoology.2018;79:8. doi:10.1186/s41936-018-0017-7.

Sakr SA, Bayomy MF, El‐Morsy AM. Rosemary extract ameliorates cadmium‐induced histological changes and oxidative damage in the liver of albino rat. The Journal of Basic and Applied Zoology.2015;71:1-9. doi:10.1016/j.jobaz.2015.01.002.

Skipper A, Sims JN, Yedjou CG, Tchounwou PB. Cadmium chloride induces DNA damage and apoptosis of human liver carcinoma cells via oxidative stress. International Journal of Environmental Research and Public Health.2016;13:88. doi:10.3390/ijerph13010088.

Chaudhary S, Iram S, Raisuddin S, Parvez S. Manganese pretreatment attenuates cadmium induced hepatotoxicity in Swiss albino mice. Trace Elem. Med. Biol. 2015; 29:284–288. doi:10.1016/j.jtemb.2014.06.013.

El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB. Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. Trace Elem. Med. Biol.2015;29:104-110. doi:10.1016/j.jtemb.2014.05.009.

Amamou F, Nemmiche S, Meziane RK, Didi A, Yazit SA, Chabane-Sari D. Protective effect of olive oil and colocynth oil against cadmium-induced oxidative stress in the liver of Wistar rats. Food Chem. Toxicol. 2015;78:177-184. doi:10.1016/j.fct.2015.01.001.

Wongmekiat O, Peerapanyasut W, Kobroob A. Catechin supplementation prevents kidney damage in rats repeatedly exposed to cadmium through mitochondrial protection. Naunyn-Schmiedeberg's Archives of Pharmacology.2018;391(4):385-394. doi: 10.1007/s00210-018-1468-6.

Ansari MA, Raish M, Ahmad A, Alkharfy KM, Ahmad SF, Attia SM, Alsaad AM, Bakheet SA. Sinapic acid ameliorate cadmium-induced nephrotoxicity: in vivo possible involvement of oxidative stress, apoptosis, and inflammation via NF-κB downregulation. Environmental toxicology and pharmacology. 2017Apr 1;51:100-7. doi:10.1016/j.etap.2017.02.014.


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Published
2021-10-05
How to Cite
Ilesanmi, O. B., & Lawal, R. A. (2021). The beneficial effect of a phytonutrient-rich product against cadmium chloride-induced hepatorenal toxicity. Ukrainian Journal of Nephrology and Dialysis, (4(72), 26-35. https://doi.org/10.31450/ukrjnd.4(72).2021.04
Issue
No 4(72) (2021): Ukrainian Journal of Nephrology and Dialysis
Section
Original Papers
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