MORPHOLOGICAL AND FUNCTIONAL STATE OF ENDOTHELIUM AT PATIENTS WITH CORONARY ARTERY DISEASE AND CHRONIC GLOMERULONEFRITIS
Abstract
All over the world the increase in quantity of patients with vascular complications of chronic inflammatory diseases of kidneys is marked. The interrelation of morphological and biochemical markers of atherosclerotic lesions were studied in 41 patients with coronary artery disease (CAD) (group 1) and 62 - with CAD and chronic glomerulonephritis (CGN) (group 2). An extracranial carotid artery (CA) status was investigated by duplex ultrasonography. VE-cadherin content determined by ELISA. States of the antioxidant system was evaluated by the concentration of malondialdehyde (MDA) and sulfhydryl groups (SH-groups) in the blood plasma by the photometric method. The levels of nitric oxide stable metabolites – NO2 and total (NO2+NO3) in plasma was determined by the photometric method. There was found that the frequency of CA plaques detection in groups 1 and 2 did not differ (27,6% and 31,0%, respectively), but patients of group 2 were reliably younger. Pronounced VE-cadherin increase (3,6-fold, p <0,01) at the background of the deterioration of renal function (2,24 -fold GFR decrease, and urinary protein excretion reaches (0,200,98) g / day) was observed in group 2. The content of NO2 in group 2 was significantly lower than in controls, and total (NO2+NO3) was increased as compared with control (2,40-fold, P <0,01), and with patients of group 1 (in 1,86-fold, p <0.05). Increase of MDA level in patients of group 2 (2,61-fold, P<0,01) accompanied by reduction of SH groups content – by 25,2% (P <0.05) compared with control. Thus, in patients with combined pathology revealed the most pronounced disorders of endothelial integrity and function, which was worsening by imbalance in the pro-antioxidant system. Accelerated atherosclerotic vascular changes in these patients are probably related to the chronic inflammatory process.
Downloads
References
Колесник М.О. Кардіоваскулярні ускладнення у хворих на хронічну хворобу нирок ./ Колесник М.О., Лапчинська І.І., Тащук В.К., Дядик О.І., Кулизський М.В. – Київ.- 2010.- 224 с.
Практикум по биохимии /Под ред. С.Е. Северина, Т.А.Соловьевой.- М.: МГУ. – 1989 г.
Синяченко О.В., Зяблицев С.В., Чернобривцев П.А. Роль эндотелиальной дисфункции в патогенезе мезангиальных гломерулонефритов / О.В. Синяченко, С.В. Зяблицев, П.А. Чернобривцев // Укр. журнал нефрології та діалізу. – 2007. – №2(14) . – С. 36-38.
Chronic uremia stimulates LDL carbamylation and atherosclerosis / E. O. Apostolov, D. Ray, A.V. Savenka [et al.] // J. Am. Soc. Nephrol. – 2010. – Vol. 21. – P. 1852-1857.
Increased risk of cardiovascular events and mortality among non-diabetic chronic kidney disease patients with hypertensive nephropathy: the Gonryo study / M. Nakayama, T. Sato, M. Miyazaki [et al.] // Hypertens. Res. – 2011. – Vol. 34(10). – P. 1106-1110.
Peters S. A. E. Improvements in risk stratification for the occurrence of cardiovascular disease by imaging subclinical atherosclerosis: a systematic review /S. A. E. Peters, H. M. den Ruijter, M. L. Bots, and K. G. M. Moons //Heart. – 2012. - № 98(3). – Р. 177-184.
Pignoli P. Intimal plus medial thickness of thearterial wall: a direct measurement with ultrasoubd imaging / P.Pignoli, E.Tremoli, A.Poli, P.Oreste, R.Paoletti // Circulation. – 1986. – Vol. 74. – P. 1399-1406.
Stein J.H. Use of Carotid Ultrasound to Identify Subclinical Vascular Disease and Evaluate Cardiovascular Disease Risk: A Consensus Statement from the American Society of Echocardiography Carotid Intima-Media Thickness Task Force Endorsed by the Society for Vascular Medicine / J.H.Stein, C.E.Korcarz, R.T. Hurst et al. // Journal of the American Society of Echocardiography. - 2008. - Vol. 48.- P. 93-111.
Cell adhesion dynamics at endothelial junctions: VE-cadherin as a major player / D. Vestweber, M. Winderlich, G. Cagna, A.F. Nottebaum // Trends Cell Biol. – 2009. – Vol. 19(1). – P. 8-15.
Yamamoto S. Mechanisms for increased cardiovascular disease in chronic kidney dysfunction / S. Yamamoto, Valentina Kon // Curr. Opin. Nephrol. Hypertens. – 2009. – Vol. 18(3). – Р.181–188.
