Apoptosis correction in nephrotic children

  • Ie. A. Burlaka Bogomolets National Medical University https://orcid.org/0000-0001-6043-7325
  • I.V. Bagdasarova State Institution «Institute of Nephrology of the National Academy of Medical Sciences»
DOI: https://doi.org/10.31450/ukrjnd.4(64).2019.03
Keywords: treatment, tocopherol, nephrotic syndrome, HIlF-1α, Bax, Bcl-xL, NF-ĸB, immunoblotting.

Abstract

Hypoxia-related disorders play an important role in irreversible kidney tissue damage via activation of certain secondary processes, i.e. apoptosis.

This study aimed to investigate the levels of apoptosis controlling factors and nuclear transcriptional factor NF-κB in relation to the value of kidney function impairment; to evaluate possible protective effects of conventional therapy with the addition of an antioxidant agent tocopherol in pediatric patients with nephrotic syndrome.

Methods. An examination of blood samples of 53 patients (aged 10 to 15 years) with nephrotic syndrome was performed. Conventional clinical investigations, immunoblotting were used in this study.

Results. We demonstrate that nephrotic patients reveal a high level of pro-apoptotic marker Bax, low level of anti-apoptotic factor BcL-xL and anti-apoptotic unit of NF-κB (p65). Their dependence on level of kidney function impairment was detected.  Applied treatment composes conventional scheme and tocopherol shows a restored balance of apoptosis controlling factor and NF-κB activity.  

Conclusion. Hypoxia-induces disorders in nephrotic children resulted in apoptosis controlling system disturbances can be attenuated by the antioxidants application.

 

Downloads

Download data is not yet available.

References

1. Eddy AA, Symons JM. Nephrotic syndrome in childhood. Lancet 2003; 362(9384):629-639. doi:10.1016/S0140-6736(03)14184-0.

2. Hemmelgarn BR, Manns BJ, Lloyd A, James MT, Klarenbach S. Relation between kidney function, proteinuria, and adverse outcomes. JAMA 2010;303:423-429. doi:10.1001/jama.2010.39

3. Certikova-Chabova V, Tesar V. Recent insights into the pathogenesis of nephrotic syndrome. Minerva Med 2013; 104(3): 333-347.

4. Christman JW, Blackwell TS, Juurlink BHJ. Redox Regulation of Nuclear Factor Kappa B: Therapeutic Potential for Attenuating Inflammatory Responses. Brain Pathology 2000;10:153-62. doi: 10.1111/j.1750-3639.2000.tb00252.x

5. Nagel M, Vincendeau M, Eitelhuber AC, Krappmann D. Mechanisms and consequences of constitutive NF-κB activation in B-cell lymphoid malignancies. Oncogene 2014;33:5655–65. doi: 10.1038/onc.2013.565.

6. KDIGO 2012 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease. Kidney Intern Suppl 2013;3:5–14. doi:10.1038/kisup.2012.77.

7. Maidannyk V, Burlaka I. Management of Hypoxia-induced Disorders in Nephrotic Children. J Cytol Histol 2015;6:362. doi:10.4172/2157-7099. 1000362.

8. Gordillo R, Spitzer A. The Nephrotic Syndrome. Pediatrics in Review 2009; 30: 94-105. doi: 10.1542/pir.30-3-94.

9. Bento CF, Pereira P. Regulation of hypoxia-inducible factor 1 and the loss of the cellular response to hypoxia in diabetes. Diabetologia 2011; 54:1946-56. doi: 10.1007/s00125-011-2191-8.

10. Eltzschig HK, Carmeliet P. Hypoxia and inflammation. N Engl J Med 2011;364(7):656-65. doi: 10.1056/NEJMra0910283.

11.  Du R, Xia L, Ning X, Liu L, Sun W, Huang C, Wang H, Sun S. Hypoxia-induced Bmi1 promotes renal tubular epithelial cell-mesenchymal transition and renal fibrosis via PI3K/Akt signal. Mol Biol Cell. 2014 Sep 1;25(17):2650-9. doi: 10.1091/mbc.E14-01-0044.

12. Volm M, Koomägi R. Hypoxia-inducible factor (HIF-1) and its relationship to apoptosis and proliferation in lung cancer. Anticancer Res. 2000;20(3A):1527-33.

13. Bin Q, Hu X, Cao Y, Gao F. The role of vitamin E (tocopherol) s Hemmelgarn B.R., Manns B.J., Lloyd A., James M.T., Klarenbach S. Relation between kidney function, proteinuria, and adverse outcomes. JAMA 2010;303:423-29. doi: 10.1001/jama.2010.39.

14.  Young JF, Rosenvold K, Stagsted J, Nielsen JH, Andersen HJ. Significance of vitamin E supplementation, dietary content of polyunsaturated fatty acids, and preslaughter stress on oxidative status in pig as reflected in cell integrity and antioxidative enzyme activities in porcine muscle. J Agric Food Chem 2005; 53(3):745-49. doi: 10.1021/jf0490652.

15. Bhatti FU, Mehmood A, Latief N, Zahra S, Cho H, Khan SN, Riazuddin S. Vitamin E protects rat mesenchymal stem cells against hydrogen peroxide-induced oxidative stress in vitro and improves their therapeutic potential in surgically-induced rat model of osteoarthritis. Osteoarthritis Cartilage. 2017;25(2):321-31. doi: 10.1016/j.joca.2016.09.014


Abstract views: 443
PDF Downloads: 17682
Published
2019-12-23
How to Cite
Burlaka, I. A., & Bagdasarova, I. (2019). Apoptosis correction in nephrotic children. Ukrainian Journal of Nephrology and Dialysis, (4(64), 15-19. https://doi.org/10.31450/ukrjnd.4(64).2019.03
Issue
No 4(64) (2019): Ukrainian Journal of Nephrology and Dialysis
Section
Original Papers