Molecular factors predicting steroid resistance in pediatric nephrotic syndrome

DOI: https://doi.org/10.31450/ukrjnd.2(70).2021.04
Keywords: treatment, nephrotic syndrome, HIF-1α, BcL-xL, steroid-resistance

Abstract

Objectives: the objective of this paper was to study the levels of cellular hypoxia, apoptosis controlling factors in children with steroid-sensitive and steroid-resistant nephrotic syndrome.

Background: patients with steroid-resistant nephrotic syndrome (SRNS) represent a challenging subset of patients with nephrotic syndrome who often fail standard immunosuppression and have a higher likelihood of progressing to end-stage renal disease. The search of the biochemical markers undergoing the steroid-resistance is under urgent need.

Methods: an examination of kidney biopsies and blood of 56 patients (aged 10 to 15 years) with nephrotic syndrome was done. Conventional clinical investigations, immunohistochemistry, immunoblotting were used in this study.

Results: patients with steroid-resistant nephrotic syndrome show an increased level of HIF-1 alfa (a marker of cellular hypoxia) as compared to the control group and children with steroid-sensitive nephrotic syndrome. Patients with steroid-resistant nephrotic syndrome show a down-regulation of anti-apoptotic marker BcL-xL as compared to the control group and children with steroid-sensitive nephrotic syndrome.

Conclusion: hypoxia-induces disorders and apoptosis activation markers are considered to be included in the complex scheme predicting steroid-resistance in nephrotic children. 

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Published
2021-03-21
How to Cite
Burlaka, I. A., & Bagdasarova, I. V. (2021). Molecular factors predicting steroid resistance in pediatric nephrotic syndrome. Ukrainian Journal of Nephrology and Dialysis, (2(70), 32-37. https://doi.org/10.31450/ukrjnd.2(70).2021.04
Issue
No 2(70) (2021): Ukrainian Journal of Nephrology and Dialysis
Section
Original Papers